Preferred learners
- Anywhere
- Academic experience
Categories
Project scope
- What is the main goal for this project?
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The objective of this project is for the learner to isolate a fungal or bacterial pathogen from an infected plant sample (BSL-1). This sample has been provided by one of our curious clients. The learner will then use universal target sequencing (ITS, 16S, CPN60, etc.) on a nanopore to identify the causative pathogen.
- What tasks will learners need to complete to achieve the project goal?
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- The learner will need to research possible pathogens that could be present in the sample. They will then need to create an experimental plan to isolate the pathogen (~20 hours).
- The learner will then grow/isolate the pathogen using the experimental plan that they designed to obtain an isolate (see photo for an example of an isolate) (~35 hours).
- The learner will prepare the sample for universal target sequencing (~5 hours).
- What is the main goal for this project?
-
The objective of this project is for the learner to isolate a fungal or bacterial pathogen from an infected plant sample (BSL-1). This sample has been provided by one of our curious clients. The learner will then use universal target sequencing (ITS, 16S, CPN60, etc.) on a nanopore to identify the causative pathogen.
- What tasks will learners need to complete to achieve the project goal?
-
- The learner will need to research possible pathogens that could be present in the sample. They will then need to create an experimental plan to isolate the pathogen (~20 hours).
- The learner will then grow/isolate the pathogen using the experimental plan that they designed to obtain an isolate (see photo for an example of an isolate) (~35 hours).
- The learner will prepare the sample for universal target sequencing (~5 hours).
- How will you support learners in completing the project?
-
The student will have full access to the Pathoscan staff to inform their design of the experiment. They will also receive full access to the Pathoscan facility to facilitate growing the cell culture. The Pathoscan staff will provide on-site biosafety training, and will supervise the student through the course of their work.
- What skills or technologies will help learners to complete the project?
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The student should have experience in growing a cell culture. The student should also have some experience with traditional PCR, and have rudimentary bioinformatics experience to interpret the nanopore results.
Supported causes
About the company
- https://pathoscan.com
- 2 - 10 employees
- Agriculture
PathoScan is a startup focused on revolutionizing agricultural practices with its innovative crop disease detection device. By allowing on-site and immediate identification of diseases, the device eliminates the need for centralized lab facilities and the associated delays. This transformative approach not only reduces costs but also empowers farmers with timely decision-making tools to ensure optimal crop health.