qPCR Laboratory Project
Preferred learners
- Saskatoon, Saskatchewan, Canada
- Academic experience
Categories
Skills
Project scope
- What is the main goal for this project?
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The goal of this project will be for the student to develop a quantitative qPCR assay for plant pathogens. This will involve taking our existing diagnostic kits and using them on spiked plasmid/amplicon samples to develop a standard curve. Field plants will then be compared to this, and an LOD/LOQ will be developed. This will be used along with a positive control to yield information about the level of infection in field crops.
- What tasks will learners need to complete to achieve the project goal?
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- The learner will need to generate a standard curve using amplicon or plasmid DNA (~5 hours).
- The learner will use this standard on the diagnostic device. (~10 hours).
- The learner will write a report and preform statistical analysis on the standard curve to generate an LOD/LOQ (~5 hours).
- The learner will run plant samples on the assay and will use the previous results to generate a copy number of pathogen per plant (~20 hours).
- The learner will repeat task four but using traditional qPCR. They will then compare these results to the ones obtained in objective 4 (~20 hours).
- What is the main goal for this project?
-
The goal of this project will be for the student to develop a quantitative qPCR assay for plant pathogens. This will involve taking our existing diagnostic kits and using them on spiked plasmid/amplicon samples to develop a standard curve. Field plants will then be compared to this, and an LOD/LOQ will be developed. This will be used along with a positive control to yield information about the level of infection in field crops.
- What tasks will learners need to complete to achieve the project goal?
-
- The learner will need to generate a standard curve using amplicon or plasmid DNA (~5 hours).
- The learner will use this standard on the diagnostic device. (~10 hours).
- The learner will write a report and preform statistical analysis on the standard curve to generate an LOD/LOQ (~5 hours).
- The learner will run plant samples on the assay and will use the previous results to generate a copy number of pathogen per plant (~20 hours).
- The learner will repeat task four but using traditional qPCR. They will then compare these results to the ones obtained in objective 4 (~20 hours).
- How will you support learners in completing the project?
-
The student will have access to a Bioinformatician to inform their LOQ/LOD calculations. They will also have full access to the Pathoscan staff, who will provide laboratory training for them and will monitor their work. Our laboratory also hosts other companies, so the learner will get to experience a dynamic real-world work environment.
Supported causes
About the company
- https://pathoscan.com
- 2 - 10 employees
- Agriculture
PathoScan is a startup focused on revolutionizing agricultural practices with its innovative crop disease detection device. By allowing on-site and immediate identification of diseases, the device eliminates the need for centralized lab facilities and the associated delays. This transformative approach not only reduces costs but also empowers farmers with timely decision-making tools to ensure optimal crop health.
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